Fluorescence sensing platform based on ruthenium(II) complexes as high 3S (sensitivity, specificity, speed) and "on-off-on" sensors for the miR-185 detection.

Title Fluorescence sensing platform based on ruthenium(II) complexes as high 3S (sensitivity, specificity, speed) and "on-off-on" sensors for the miR-185 detection.
Authors B. Sun; Z. Liang; B.P. Xie; R.T. Li; L.Z. Li; Z.H. Jiang; L.P. Bai; J.X. Chen
Journal Talanta
DOI 10.1016/j.talanta.2017.11.047
Abstract

Inspired by the enormous importance attributed to the biological function of miRNA, we pour our attention into the design and synthesis of four ruthenium(II) complexes and evaluate their applications as miR-185 detection agents by spectroscopic measurements. It was found that all complexes can form sensing platform for the detection of the complementary target miR-185 through the introduction of carboxyfluorescein (FAM) labeled single stranded DNA (P-DNA), giving the detection limits of 0.42nM for Ru 1, 0.28nM for Ru 2, 0.32nM for Ru 3, 0.85nM for Ru 4, all with instantaneous detection time in 1min. The results of the binding constant, fluorescence anisotropy (FA) and polyacrylamide gel electrophoresis experiments (PAGE) revealed that the ruthenium(II) complexes prefer to bind P-DNA other than hybrid duplexes DNA@RNA upon recognition, resulting in the detection of miR-185. These results provide useful suggestions in the new type of metal-based miRNA detection agents.

Citation B. Sun; Z. Liang; B.P. Xie; R.T. Li; L.Z. Li; Z.H. Jiang; L.P. Bai; J.X. Chen.Fluorescence sensing platform based on ruthenium(II) complexes as high 3S (sensitivity, specificity, speed) and "on-off-on" sensors for the miR-185 detection.. Talanta. 2018;179:658667. doi:10.1016/j.talanta.2017.11.047

Related Elements

Ruthenium

See more Ruthenium products. Ruthenium (atomic symbol: Ru, atomic number: 44) is a Block D, Group 8, Period 5 element with an atomic weight of 101.07. Ruthenium Bohr ModelThe number of electrons in each of ruthenium's shells is [2, 8, 18, 15, 1] and its electron configuration is [Kr] 4d7 5s1. The ruthenium atom has a radius of 134 pm and a Van der Waals radius of 207 pm. Ruthenium was discovered by Jędrzej Śniadecki in 1807. It was first recognized as a distinct element by Karl Ernst Claus in 1844. Elemental RutheniumIn its elemental form, ruthenium has a silvery white metallic appearance. Ruthenium is a rare transition metal belonging to the platinum group of metals. It is found in pentlandite, pyroxenite, and platinum group metal ores. The name Ruthenium originates from the Latin word "Ruthenia," meaning Russia.

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