Lanthanum Chloride Inhibits LPS Mediated Expressions of Pro-Inflammatory Cytokines and Adhesion Molecules in HUVECs: Involvement of NF-?B-Jmjd3 Signaling.

Title Lanthanum Chloride Inhibits LPS Mediated Expressions of Pro-Inflammatory Cytokines and Adhesion Molecules in HUVECs: Involvement of NF-?B-Jmjd3 Signaling.
Authors X. Chen; M. Xiu; J. Xing; S. Yu; D. Min; F. Guo
Journal Cell Physiol Biochem
DOI 10.1159/000479439
Abstract

BACKGROUND/AIMS: To investigate the regulation of LaCl3 on lipopolysaccharides (LPS)-induced pro-inflammatory cytokines and adhesion molecules in human umbilical vein endothelial cells (HUVECs).

METHODS: Primary cultured HUVECs were pretreated with 2.5 µM LaCl3 for 30 min followed by 1 µg/ml LPS for 2 h. Pro-inflammatory cytokine and adhesion molecule expressions were determined by real-time RT-PCR and ELISA. NF-?B/p65 nuclear translocation was examined by immunofluorescence and immuno-blot, and its DNA-binding activity was measured by chemiluminescence. Recruitment of NF-?B/p65, Jmjd3, and H3K27me3 to gene promoter regions was determined by ChIP-qPCR.

RESULTS: LaCl3 exhibited no cytotoxic effects to primary HUVECs at concentrations ? 50 µM. LPS-mediated TNF-?, IL-1?, IL-6, MMP-9, and ICAM-1 production, nuclear translocation, and DNA-binding activity of NF-?B/p65, as well as Jmjd3 expression, were all reduced significantly by LaCl3. Furthermore, LaCl3 treatment significantly impaired LPS-induced enrichment of NF-?B/p65 to the promoter regions of TNF-?, MMP-9, IL-1?, ICAM-1, and IL-6; and of Jmjd3 to the promoter regions of TNF-?, MMP-9, IL-1?, and IL-6. H3K27me3 abundance in the promoter regions of TNF-? and ICAM-1 increased significantly in following LaCl3 treatment.

CONCLUSION: LaCl3 inhibits pro-inflammatory cytokine and adhesion molecule expressions induced by LPS in HUVECs. NF-?B and histone demethylase Jmjd3 are involved in this effect.

Citation X. Chen; M. Xiu; J. Xing; S. Yu; D. Min; F. Guo.Lanthanum Chloride Inhibits LPS Mediated Expressions of Pro-Inflammatory Cytokines and Adhesion Molecules in HUVECs: Involvement of NF-?B-Jmjd3 Signaling.. Cell Physiol Biochem. 2017;42(5):17131724. doi:10.1159/000479439

Related Elements

Chlorine

17 Cl 35.453000000 Chlorine

Chlorine is a Block P, Group 17, Period 3 element. Its electron configuration is [Ne]3s23p5. The chlorine atom has a covalent radius of 102±4 pm and its Van der Waals radius is 175 pm. Chlorine ModelIn its elemental form, chlorine is a yellow-green gas. Chlorine is the second lightest halogen after fluorine. It has the third highest electronegativity and the highest electron affinity of all elements, making it a strong oxidizing agent. It is rarely found by itself in nature. Chlorine was discovered and first isolated by Carl Wilhelm Scheele in 1774. It was first recognized as an element by Humphry Davy in 1808.

Lanthanum

57 La 138.905470000 Lanthanum

See more Lanthanum products. Lanthanum (atomic symbol: La, atomic number: 57) is a Block F, Group 3, Period 6 element with an atomic weight of 138.90547. Lanthanum Bohr ModelThe number of electrons in each of lanthanum's shells is [2, 8, 18, 18, 9, 2] and its electron configuration is [Xe] 5d1 6s2. The lanthanum atom has a radius of 187 pm and a Van der Waals radius of 240 pm. Lanthanum was first discovered by Carl Mosander in 1838. In its elemental form, lanthanum has a silvery white appearance.Elemental Lanthanum It is a soft, malleable, and ductile metal that oxidizes easily in air. Lanthanum is the first element in the rare earth or lanthanide series. It is the model for all the other trivalent rare earths and it is the second most abundant of the rare earths after cerium. Lanthanum is found in minerals such as monazite and bastnasite. The name lanthanum originates from the Greek word Lanthaneia, which means 'to lie hidden'.

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