Optimization of conditions for cadmium selenide quantum dot biosynthesis in Saccharomyces cerevisiae.

Title Optimization of conditions for cadmium selenide quantum dot biosynthesis in Saccharomyces cerevisiae.
Authors J. Brooks; D.D. Lefebvre
Journal Appl Microbiol Biotechnol
DOI 10.1007/s00253-016-8056-9
Abstract

The biosynthesis of quantum dots has been explored as an alternative to traditional physicochemical methods; however, relatively few studies have determined optimal synthesis parameters. Saccharomyces cerevisiae sequentially treated with sodium selenite and cadmium chloride synthesized CdSe quantum dots in the cytoplasm. These nanoparticles displayed a prominent yellow fluorescence, with an emission maximum of approximately 540 nm. The requirement for glutathione in the biosynthetic mechanism was explored by depleting its intracellular content through cellular treatments with 1-chloro-2,4-dinitrobenzene and buthionine sulfoximine. Synthesis was significantly inhibited by both of these reagents when they were applied after selenite treatment prior to the addition of cadmium, thereby indicating that glutathione contributes to the biosynthetic process. Determining the optimum conditions for biosynthesis revealed that quantum dots were produced most efficiently at entry into stationary phase followed by direct addition of 1 mM selenite for only 6 h and then immediately incubating these cells in fresh growth medium containing 3 mM Cd (II). Synthesis of quantum dots reached a maximum at 84 h of reaction time. Biosynthesis of 800-?g g(-1) fresh weight cells was achieved. For the first time, significant efforts have been undertaken to optimize each aspect of the CdSe biosynthetic procedure in S. cerevisiae, resulting in a 70% increased production.

Citation J. Brooks; D.D. Lefebvre.Optimization of conditions for cadmium selenide quantum dot biosynthesis in Saccharomyces cerevisiae.. Appl Microbiol Biotechnol. 2017;101(7):27352745. doi:10.1007/s00253-016-8056-9

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Selenium

Selenium Bohr ModelSee more Selenium products. Selenium (atomic symbol: Se, atomic number: 34) is a Block P, Group 16, Period 4 element with an atomic radius of 78.96. The number of electrons in each of Selenium's shells is 2, 8, 18, 6 and its electron configuration is [Ar] 3d10 4s2 4p4. The selenium atom has a radius of 120 pm and a Van der Waals radius of 190 pm. Selenium is a non-metal with several allotropes: a black, vitreous form with an irregular crystal structure three red-colored forms with monoclinic crystal structures and a gray form with a hexagonal crystal structure, the most stable and dense form of the element. Elemental SeleniumOne of the most common uses for selenium is in glass production the red tint that it lends to glass neutralizes green or yellow tints from impurities in the glass materials. Selenium was discovered and first isolated by Jöns Jakob Berzelius and Johann Gottlieb Gahn in 1817. The origin of the name Selenium comes from the Greek word "Selênê," meaning moon.

Cadmium

See more Cadmium products. Cadmium (atomic symbol: Cd, atomic number: 48) is a Block D, Group 12, Period 5 element with an atomic weight of 112.411. Cadmium Bohr ModelThe number of electrons in each of Cadmium's shells is 2, 8, 18, 18, 2 and its electron configuration is [Kr]4d10 5s2. The cadmium atom has a radius of 151 pm and a Van der Waals radius of 230 pm. Cadmium was discovered and first isolated by Karl Samuel Leberecht Hermann and Friedrich Stromeyer in 1817. In its elemental form, cadmium has a silvery bluish gray metallic appearance. Cadmium makes up about 0.1 ppm of the earth's crust. Elemental CadmiumNo significant deposits of cadmium containing ores are known, however, it is sometimes found in its metallic form. It is a common impurity in zinc ores and is isolated during the production of zinc. Cadmium is a key component in battery production and particular pigments and coatings due to its distinct yellow color. Cadmium oxide is used in phosphors for television picture tubes. The name Cadmium originates from the Latin word 'cadmia' and the Greek word 'kadmeia'.

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