Performance of a MALDI-TOF MS-based imipenem hydrolysis assay incorporating zinc sulfate.

Title Performance of a MALDI-TOF MS-based imipenem hydrolysis assay incorporating zinc sulfate.
Authors J. Knox; E. Palombo
Journal Diagn Microbiol Infect Dis
DOI 10.1016/j.diagmicrobio.2016.11.018
Abstract

A MALDI-TOF MS(1)-based imipenem hydrolysis assay was modified by adding ZnSO4. This improved detection of metallo-?-lactamase producing strains without compromising detection of other carbapenemase types. Using 129 genetically characterized Gram-negative bacilli, the sensitivity and specificity were 98.5% (95% confidence interval [CI]: 91.9-99.7%) and 100% (95% CI: 94.3-100%), respectively.

Citation J. Knox; E. Palombo.Performance of a MALDI-TOF MS-based imipenem hydrolysis assay incorporating zinc sulfate.. Diagn Microbiol Infect Dis. 2017;87(3):258260. doi:10.1016/j.diagmicrobio.2016.11.018

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Zinc

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