3D flower-like ferrous(II) phosphate nanostructures as peroxidase mimetics for sensitive colorimetric detection of hydrogen peroxide and glucose at nanomolar level.

Author(s) Guo, J.; Wang, Y.; Zhao, M.
Journal Talanta
Date Published 2018 May 15
Abstract

Ferrous(II) phosphate nanoflowers (Fe(PO)·8HO NFs) were synthesized by a facial co-precipitation method. The structure, composition and morphology were characterized by XRD, EDX, element mapping and FESEM. The as-prepared Fe(PO)·8HO NFs exhibited excellent intrinsic peroxidase-like activity. Steady-state kinetic studies showed that Fe(PO)·8HO NFs exhibited stronger affinities with 3,3,5,5-tetramethylbenzidine (TMB) and HOas the substrates compared with the natural horseradish peroxidase (HRP) and the catalytic constant (k) value was even higher than HRP and other reported nanomaterial based peroxidase mimics. The investigation of the catalytic mechanism by cyclic voltammetry, fluorescence spectroscopy and ESR displayed the catalytic activity of Fe(PO)·8HO NFs originated from the generation of •OH. The Fe(PO)·8HO NFs also exhibited higher robustness and better storage stability than HRP. Then, a Fe(PO)·8HO NFs-based colorimetric platform was developed to determine HOand glucose. The linear range of HOand glucose was as broad as 1 × 10-2.5 mM and 8 × 10-1.2 mM, and the detection limit (LOD) was as low as 5 nM and 35 nM, respectively. This simple assay offered a highly sensitive and specific strategy for HOand glucose determination, which had been successfully utilized for real sample analysis with good reproducibility and accuracy.

DOI 10.1016/j.talanta.2018.01.080
ISSN 1873-3573
Citation Guo J, Wang Y, Zhao M. 3D flower-like ferrous(II) phosphate nanostructures as peroxidase mimetics for sensitive colorimetric detection of hydrogen peroxide and glucose at nanomolar level. Talanta. 2018;182:230-240.

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