A novel ratiometric fluorescent probe for the detection of uric acid in human blood based on HO-mediated fluorescence quenching of gold/silver nanoclusters.

Author(s) Wang, X.Y.; Zhu, G.B.; di Cao, W.; Liu, Z.J.; Pan, C.G.; Hu, W.J.; Zhao, W.Y.; Sun, J.F.
Journal Talanta
Date Published 2019 Jan 01
Abstract

In this work, a ratiometric fluorescent probe (RF-probe) for highly sensitive and selective detection of uric acid was reported for the first time toward HO based on inner filter effect (IFE) between bimetallic gold/silver nanoclusters (Au/Ag NCs) and 2,3-diaminophenazine (DAP). For this RF-probe, uric acid was degraded to allantoin and HO. Upon the addition of HRP, o-phenylenediamine (OPD) could be catalytically oxidized to DAP in the presence of HO, then the fluorescence intensity corresponding to DAP at 580 nm increased dramatically with a fluorescence quenching of BSA-Au/Ag NCs at 690 nm, resulting in a RF-probe toward uric acid. This RF-probe allowed for the sensitive detection of uric acid in range of 5.0 × 10 M to 5.0 × 10 M with a detection limit (S/N = 3) as low as 5.1 × 10 M. At the same time, it has been successfully used for uric acid levels detection in human serum, and the results are consistent with those of the hospital. RF-probe built may provide a ratiometric fluorescence universal platform for detection of various species involving in the production of HO in other biological systems.

DOI 10.1016/j.talanta.2018.08.015
ISSN 1873-3573
Citation Wang X-, Zhu G-, di Cao W-, Liu Z-, Pan C-, Hu W-, et al. A novel ratiometric fluorescent probe for the detection of uric acid in human blood based on HO-mediated fluorescence quenching of gold/silver nanoclusters. Talanta. 2019;191:46-53.

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