An electrooxidative technique to fast fabricate copper phosphate electrodes capable of integrating high performance liquid chromatography for the label-free detection of fish freshness.

Author(s) Hsieh, Y.H.; Lee, M.Y.; Wu, C.C.
Journal Food Chem
Date Published 2018 Dec 15
Abstract

A simple and fast one-step electrooxidative method has been developed to monolithically produce a copper phosphate (Cu(PO)) compound on a disposable copper tape, which can be integrated with high performance liquid chromatography (HPLC) for the estimation of fish freshness. The Cu(PO) compound of flake-like nanostructures was formed by applying a first anodic peak potential at the copper tape for 10 min in a 1 M sodium dihydrogen phosphate (NaHPO) (pH 5.0) solution. The Cu(PO) electrodes can detect the oxidative reaction of histidine and histamine in 20 mM NaHPO solutions with pH 5.0-8.5. When integrating the electrodes with a flow injection system, the linear range and the calculated detection limit of histamine were respectively 2.5-250 ppm and 0.15 ppm. The electrodes integrated to HPLC can specifically detect the histamine concentrations in fish samples in the pH 7.5 NaHPO solution, achieving an accuracy rate of 95.3% and a recovery rate of 101.1%.

DOI 10.1016/j.foodchem.2018.06.125
ISSN 0308-8146
Citation Hsieh Y-, Lee M-, Wu C-. An electrooxidative technique to fast fabricate copper phosphate electrodes capable of integrating high performance liquid chromatography for the label-free detection of fish freshness. Food Chem. 2018;269:16-23.

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