An Study of Bactericidal Effect of Gallium Aluminium Arsenide Laser on Anaerobic Photosensitized Periodontopathics.

Author(s) Dodani, K.; Khare, N.; Bathini, C.; Mishra, S.; Inamdar, M.Nk; Nasha, A.
Journal J Contemp Dent Pract
Date Published 2019 Mar 01

AIM: To investigate the bactericidal effect of gallium aluminium arsenide (GaAlAs) laser on photosensitized predominant periodontopathogenic anaerobic organisms.

MATERIALS AND METHODS: The present prospective study was carried out among 50 patients of chronic periodontitis with pockets ≥ 5 mm depth. For sample analysis of and , a subgingival plaque was collected from periodontal pockets using sterile curettes under aseptic conditions. The sample was then immediately transported to microbiology laboratory using Transport media, Thioglycollate broth with Vitamin K and Hemin. After incubation period again the number of the viable bacterial count was carried out using a magnifying glass and expressed as CFU/mL, to determine the bactericidal effect of GaAlAs laser. A predetermined number of colonies (for 500 colonies were taken initially; for 400 colonies were taken initially) were taken equally as control group and case group. The data was statistically analyzed using the SPSS statistical software version 16. Mann-Whitney Test was used for statistical analysis with value less than 0.05 considered statically significant.

RESULTS: Study group shows a reduction in colony count of after being exposed to GaAlAs diode laser for 1 minute from 369-229, 134 and 41 which was statistically significant with value <0.0001. Study group shows a decrease in colony count of after being exposed to GaAlAs diode laser for 1 minute from 286.52-197, 94 and 39 which was statistically significant with value <0.0001.

CONCLUSION: The result of our study implies that for all tested bacterial strains, the effect of GaAlAs laser for 1 minute resulted in a significant reduction in the viable counts of photosensitized predominant periodontopathogenic anaerobic organisms. Photodynamic therapy (PDT) kills the bacteria and also leads to the detoxification of endotoxins. Further, it can thus be safely concluded that this technique is an alternative method adjunct to mechanical therapy.

CLINICAL SIGNIFICANCE: The application of an alternative method to eradicate bacteria from periodontal pockets is desirable. One such approach is photodynamic therapy (PDT), i.e., laser therapy. Owing to the emergence of antibiotic resistance, PDT has to turn out to be a feasible alternative antibacterial therapy for biofilm-related diseases.

Keywords Anaerobiosis; Anti-Bacterial Agents; Humans; Lasers, Semiconductor; Photochemotherapy; Porphyromonas gingivalis; Prospective Studies
ISSN 1526-3711
Citation J Contemp Dent Pract. 2019;20(3):385389.

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