Cerium chloride stimulated controlled conversion of B-to-Z DNA in self-assembled nanostructures.

Author(s) Bhanjadeo, M.M.; Nayak, A.K.; Subudhi, U.
Journal Biochem Biophys Res Commun
Date Published 2017 Jan 22

DNA adopts different conformation not only because of novel base pairs but also while interacting with inorganic or organic compounds. Self-assembled branched DNA (bDNA) structures or DNA origami that change conformation in response to environmental cues hold great promises in sensing and actuation at the nanoscale. Recently, the B-Z transition in DNA is being explored to design various nanomechanical devices. In this communication we have demonstrated that Cerium chloride binds to the phosphate backbone of self-assembled bDNA structure and induce B-to-Z transition at physiological concentration. The mechanism of controlled conversion from right-handed to left-handed has been assayed by various dye binding studies using CD and fluorescence spectroscopy. Three different bDNA structures have been identified to display B-Z transition. This approach provides a rapid and reversible means to change bDNA conformation, which can be used for dynamic and progressive control at the nanoscale.

DOI 10.1016/j.bbrc.2016.11.133
ISSN 1090-2104
Citation Biochem Biophys Res Commun. 2017;482(4):916921.

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