Author(s) Balagangadharan, K.; Chandran, V.; Arumugam, B.; Saravanan, S.; Venkatasubbu, D.; Selvamurugan, N.
Journal Int J Biol Macromol
Date Published 2018 May

Bone tissue engineering (BTE) relies on biocomposite scaffolds and bioactive molecules for bone regeneration. The present study was aimed to synthesize and characterize biocomposite scaffolds containing chitosan (CS), nano-hydroxyapatite (nHAp) and nano‑zirconium dioxide (nZrO) along with microRNA (miRNA) for BTE applications. miRNAs act as post-transcriptional regulator of gene expression. The fabricated biocomposite scaffolds were characterized using SEM, FT-IR and XRD analyses. The effect of a bioactive molecule (miR-590-5p) with scaffolds was tested for osteoblast differentiation at the cellular and molecular levels using mouse mesenchymal stem cells (C3H10T1/2). The results showed that CS/nHAp/nZrO scaffolds promoted osteoblast differentiation, and this effect was further increased in the presence of miR-590-5p in C3H10T1/2 cells. Thus, we suggested that CS/nHAp/nZrO scaffolds with miR-590-5p would have potential towards the treatment of bone defects.

DOI 10.1016/j.ijbiomac.2018.01.122
ISSN 1879-0003
Citation Int J Biol Macromol. 2018;111:953958.

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