Ciprofloxacin HCl-loaded calcium carbonate nanoparticles: preparation, solid state characterization, and evaluation of antimicrobial effect against Staphylococcus aureus.

Author(s) Dizaj, S.Maleki; Lotfipour, F.; Barzegar-Jalali, M.; Zarrintan, M.H.; Adibkia, K.
Journal Artif Cells Nanomed Biotechnol
Date Published 2017 May

Ciprofloxacin HCl-loaded calcium carbonate (CaCO3) nanoparticles were prepared via a w/o microemulsion method and characterized by dynamic light scattering, scanning electron microscopy, X-ray powder diffraction (XRPD) analysis, differential scanning calorimetry (DSC), and Fourier transform infrared spectroscopy (FTIR). The in vitro drug release profiles as well as antimicrobial effect against Staphylococcus aureus (S. aureus) were also evaluated. The antibacterial effect was studied using serial dilution technique to determine the minimum inhibitory concentration (MIC) of the nanoparticles and was confirmed by streak cultures. The mean particle size, drug loading and entrapment efficiency were calculated to be 116.09 nm, 20.49% and 44.05%, respectively. PXRD and FTIR studies confirmed that both vaterite and calcite polymorphs of CaCO3 were formed during the preparation process. In vitro release profiles of the nanoparticles showed slow release pattern for 12 h. The drug-loaded nanoparticles showed similar MICs against S. aureus compared to untreated drug. However, a preserved antimicrobial effect was observed for drug-loaded nanoparticles compared to untreated drug after 2 days of incubation.

DOI 10.3109/21691401.2016.1161637
Keywords Anti-Bacterial Agents; Calcium Carbonate; Ciprofloxacin; Delayed-Action Preparations; Drug Compounding; Drug Liberation; Emulsions; Kinetics; Microbial Sensitivity Tests; Nanoparticles; Particle Size; Staphylococcus aureus
ISSN 2169-141X
Citation Artif Cells Nanomed Biotechnol. 2017;45(3):535543.

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