Author(s) Setty, M.Kumar Hale; Kurdekar, A.; Mahtani, P.; Liu, J.; Hewlett, I.
Journal AIDS Res Hum Retroviruses
Date Published 2018 Nov 09

BACKGROUND: Accurate and early detection of diverse HIV-1 subtypes using currently available assays has been a major challenge. We report development of a sensitive Time Resolved Fluorescence (TRF) Europium Nanoparticle Immuno Assay (ENIA) for cross subtype detection of p24 antigen using broadly cross reactive antibodies.

METHODS: Several antibodies were tested for optimal reactivity with antigens of diverse HIV-1 subtypes and CRFs. We tested HIV strains using this assay for sensitivity and quantification ability at the pico-gram (pg)/ml level.

RESULTS: We identified two broadly cross reactive antibodies C65690M and ANT-152, which detected all strains of HIV tested. These two antibodies also yield a better signal to cut off ratios for the same amount of antigen tested in comparison to a commercial assay.

CONCLUSION: Using a combination of appropriate C65690M and ANT-152 p24 antibodies capable of detecting all HIV types and highly sensitive time resolved fluorescence (TRF) based Europium Nano particle assay platform, we developed a sensitive p24 antigen assay that can detect HIV infection of all HIV subtypes and may be useful in early detection.

DOI 10.1089/AID.2018.0163
ISSN 1931-8405
Citation AIDS Res Hum Retroviruses. 2018.

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