Title | Designing of carbon based fluorescent nanosea-urchin via green-synthesis approach for live cell detection of zinc oxide nanoparticle. |
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Authors | Choudhary, R.; Patra, S.; Madhuri, R.; Sharma, P.K. |
Journal | Biosens Bioelectron |
DOI | 10.1016/j.bios.2016.12.067 |
Abstract |
The present work reports an eco-friendly and economic method for the preparation of different shaped (spherical, rod, and sea-urchin) heteroatom-doped fluorescent carbon nanoparticles (CNPs) by a unique combination of sonochemical, microwave and hydrothermal approach. Not only in terms of chemical, herein, we used the green precursors i.e. six members from solonaceae family for the synthesis of CNPs. Among the various shapes of CNPs, the sea-urchin shape CNPs (SU-CNPs) shows the high product and quantum yield with good photostability, excellent water dispersibility, narrow size distribution and high storage ability. The high quantum yield of derived CNPs made them suitable for patterning and staining (fluorescent inks) as a better, economic and eco-friendly replacement of dyes. In addition, the synthesized SU-CNPs were employed for quantitative detection of a widely used nanomaterial i.e. zinc oxide nanoparticles (ZnO). The fluorescence sensor was successfully applied for the detection of ZnO nanoparticles in wastewater, human sera and some cosmetic samples without any cross-reactivity. Herein, for the first time, we have successfully used the SU-CNPs for live cell detection of ZnO nanoparticles. The in vitro cytocompatibility study demonstrated that the SU-CNPs were not harmful to the cell up to a very high concentration of 1500.0mgL(-1) and could be used for cell imaging. In addition, the SU-CNPs were successfully utilized for the qualitative and quantitative, intracellular detection of ZnO nanoparticle in breast cancer cell line i.e. MCF-7. |
Designing of carbon based fluorescent nanosea-urchin via green-synthesis approach for live cell detection of zinc oxide nanoparticle.