Evaluation of the Putative Efficacy of a Methanolic Extract of in Preventing Disruption of Structural Proteins in an in Vitro System of Selenite-induced Cataractogenesis.

Author(s) Anand, T.; Anbukkarasi, M.; Teresa, A.; Thomas, P.Aloysius; Geraldine, P.
Journal Curr Eye Res
Date Published 2019 Dec 06
Abstract

: To evaluate whether a methanolic extract of Ocimum basilicum (OB) leaves prevented lenticular protein alterations in an in-vitro model of selenite-induced cataractogenesis.: Transparent lenses extirpated from Wistar rats were divided into three groups: control; selenite only; treated. Control lenses were cultured in Dulbecco's modified Eagle's medium (DMEM) alone, selenite only lenses were cultured in DMEM containing sodium selenite only (100 µM selenite/ml DMEM) and treated lenses were cultured in DMEM containing sodium selenite and the methanolic extract of OB leaves (200 µg of extract/ml DMEM); all lenses were cultured for 24 h and then processed. The parameters assessed in lenticular homogenates were lenticular protein sulfhydryl and carbonyl content, calcium level, insoluble to soluble protein ratio, sodium dodecyl sulphate-polyacrylamide gel electrophoretic (SDS-PAGE) patterns of lenticular proteins, and mRNA transcript and protein levels of αA-crystallin and βB1-crystallins.: Selenite only lenses exhibited alterations in all parameters assessed. Treated lenses exhibited values for these parameters that were comparable to those noted in normal control lenses.: The methanolic extract of OB leaves prevented alterations in lenticular protein sulfhydryl and carbonyl content, calcium level, insoluble to soluble protein ratio, SDS-PAGE patterns of lenticular proteins, and expression of αA-crystallin and βB1-crystallin gene and proteins in cultured selenite-challenged lenses. OB may be further evaluated as a promising agent for the prevention of cataract.

DOI 10.1080/02713683.2019.1698054
ISSN 1460-2202
Citation Anand T, Anbukkarasi M, P Teresa A, Thomas PA, Geraldine P. Evaluation of the Putative Efficacy of a Methanolic Extract of in Preventing Disruption of Structural Proteins in an in Vitro System of Selenite-induced Cataractogenesis. Curr Eye Res. 2019:1-9.

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