Excitation of erbium-doped nanoparticles in 1550-nm wavelength region for deep tissue imaging with reduced degradation of spatial resolution.

Author(s) Yamanaka, M.; Niioka, H.; Furukawa, T.; Nishizawa, N.
Journal J Biomed Opt
Date Published 2019 Jul
Abstract

Rare-earth-doped nanoparticles are one of the emerging probes for bioimaging due to their visible-to-near-infrared (NIR) upconversion emission via sequential single-photon absorption at NIR wavelengths. The NIR-excited upconversion property and high photostability make this probe appealing for deep tissue imaging. So far, upconversion nanoparticles include ytterbium ions (Yb3  +  ) codoped with other rare earth ions, such as erbium (Er3  +  ) and thulium (Tm3  +  ). In these types of upconversion nanoparticles, through energy transfer from Yb3  +   excited with continuous wave light at a wavelength of 980 nm, upconversion emission of the other rare earth dopants is induced. We have found that the use of the excitation of Er3  +   in the 1550-nm wavelength region allows us to perform deep tissue imaging with reduced degradation of spatial resolution. In this excitation-emission process, three and four photons of 1550-nm light are sequentially absorbed, and Er3  +   emits photons in the 550- and 660-nm wavelength regions. We demonstrate that, compared with the case using 980-nm wavelength excitation, the use of 1550-nm light enables us to moderate degradation of spatial resolution in deep tissue imaging due to the lower light scattering coefficient compared with 980-nm light. We also demonstrate that live cell imaging is feasible with this 1550 nm excitation.

DOI 10.1117/1.JBO.24.7.070501
ISSN 1560-2281
Citation Yamanaka M, Niioka H, Furukawa T, Nishizawa N. Excitation of erbium-doped nanoparticles in 1550-nm wavelength region for deep tissue imaging with reduced degradation of spatial resolution. J Biomed Opt. 2019;24(7):1-4.

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