Gadolinium borate and iron oxide bioconjugates: Nanocomposites of next generation with multifunctional applications.

Author(s) Icten, O.; Kose, D.Ali; Matissek, S.J.; Misurelli, J.A.; Elsawa, S.F.; Hosmane, N.S.; Zumreoglu-Karan, B.
Journal Mater Sci Eng C Mater Biol Appl
Date Published 2018 Nov 01

The systematic investigations concerning the bioconjugation of GdBO-FeO nanocomposite and their in vitro biocompatibility with cancer cell lines are reported. The nanocomposites were prepared hydrothermally from magnetite (FeO), borax or boric acid and a Gd salt. Bioconjugation processes were performed with citric acid and fluorescein isothiocyanate-doped silica, followed by the treatment with folic acid. Overall, the procedure involved "bare or PEGylated FeO as the magnetic core" and "vaterite- or triclinic-type of GdBO as the surface borate layer" for comparative evaluation of the results. The successful vectorization of the nanocomposite particles was demonstrated by quantitative and qualitative analytical data. All bioconjugates displayed soft ferromagnetic properties and negative zeta potential values that are appropriate for biological applications. The B and Gd contents were ca. 10 atom/μg making them promising agents for BNCT, GdNCT and the combined GdBNCT. The Gd/Fe molar ratios (0.27-0.63) provided the capability for T1- or dual (T1 + T2) magnetic resonance imaging (MRI). In vitro studies were conducted to investigate the efficiency of targeted FA-conjugated versus non-FA conjugated nanoformulations on Mia-Pa-Ca-2, HeLa and A549 cells. Fluorescence microscopy and flow cytometry data unveiled the essential role of the zeta potential competing with folate targeting in the uptake mechanism. The bioconjugated nanoplatforms of GdBO-FeO composite, introduced herein, proved to have potential features of next generation agents for magnetically targeted therapy, fluorescence imaging, magnetic resonance imaging/diagnosis and Neutron Capture Therapy.

DOI 10.1016/j.msec.2018.06.042
ISSN 1873-0191
Citation Mater Sci Eng C Mater Biol Appl. 2018;92:317328.

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