Improvement of a rapid diagnostic application of monoclonal antibodies against avian influenza H7 subtype virus using Europium nanoparticles.

Author(s) Yeo, S.J.; Bao, D.Tuan; Seo, G.E.; Bui, C.Thi; Kim, D.Thi Hoang; Anh, N.Thi Viet; Tien, T.Thi Thuy; Linh, N.Thi Phuong; Sohn, H.J.; Chong, C.K.; Shin, H.J.; Park, H.
Journal Sci Rep
Date Published 2017 Aug 11

The development of a sensitive and rapid diagnostic test is needed for early detection of avian influenza (AI) H7 subtype. In this study, novel monoclonal antibodies (mAbs) against influenza A H7N9 recombinant hemagglutinin (rHA)1 were developed and applied to a Europium nanoparticle-based rapid fluorescent immunochromatographic strip test (FICT) to improve the sensitivity of the rapid diagnostic system. Two antibodies (2F4 and 6D7) exhibited H7 subtype specificity in a dot-FICT assay by optimization of the conjugate and the pH of the lysis buffer. The subtype specificity was confirmed by an immunofluorescence assay and Western blot analysis. The limit of detection of the FICT employing novel mAbs 31 ng/mL for H7N9 rHA1 and 40 hemagglutination units/mL for H7 subtype virus. Sensitivity was improved 25-fold using Europium as confirmed by comparison of colloidal gold-based rapid diagnostic kit using the 2F4 and 6D7 mAbs.

DOI 10.1038/s41598-017-08328-9
ISSN 2045-2322
Citation Sci Rep. 2017;7(1):7933.

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