Measurement of aflatoxin M1 in powder and pasteurized milk samples by using a label-free electrochemical aptasensor based on platinum nanoparticles loaded on Fe-based metal-organic frameworks.

Author(s) Jahangiri-Dehaghani, F.; Zare, H.R.; Shekari, Z.
Journal Food Chem
Date Published 2020 Apr 25

In the present study, a sensitive label-free electrochemical aptasensor is introduced to measure aflatoxin M1 (AFM1) by using platinum nanoparticles (PtNPs) decorated on a glassy carbon electrode (GCE) modified with Fe-based metal-organic frameworks, MIL-101(Fe). The MIL-101(Fe) and the PtNP/MIL-101(Fe) are synthesized and characterized by Fourier transform infrared spectroscopy, X-ray diffraction, UV-Visible spectroscopy, and field-emission scanning electron microscopy. Cyclic voltammetry and electrochemical impedance spectroscopy (EIS) are done to monitor the fabrication processes of the aptasensor. In optimum conditions, the linear calibration range of 1.0 × 10 to 80.0 ng mL and the detection limit of 2.0 × 10 ng mL are obtained to measure AFM1 concentration using the EIS method. Finally, the fabricated aptasensor is successfully applied to measure AFM1 concentration in powder and pasteurized milk samples.

DOI 10.1016/j.foodchem.2019.125820
Keywords Aflatoxin M1; Animals; Aptamers, Peptide; Carbon; Dielectric Spectroscopy; Electrochemical Techniques; Electrodes; Food Analysis; Food Contamination; Iron; Limit of Detection; Metal Nanoparticles; Metal-Organic Frameworks; Milk; Pasteurization; Platinum; Powders; Spectroscopy, Fourier Transform Infrared; X-Ray Diffraction
ISSN 1873-7072
Citation Food Chem. 2020;310:125820.