Mito-Specific Ratiometric Terbium(III)-Complex-Based Luminescent Probe for Accurate Detection of Endogenous Peroxynitrite by Time-Resolved Luminescence Assay.

Author(s) Wang, Y.; Li, B.; Song, X.; Shen, R.; Wang, D.; Yang, Y.; Feng, Y.; Cao, C.; Zhang, G.; Liu, W.
Journal Anal Chem
Date Published 2019 Oct 01
Abstract

The level of peroxynitrite (ONOO) is closely related to the pathogenesis of a series of diseases. Hence, the accurate sensing of ONOO is very important for a better understanding of its biological functions. Here, a time-resolved ratiometric nanoprobe (TD-DC) composed of an energy donor unit (Tb(DPA), where DPA signifies a dipicolinate dianion ligand) and an energy receptor unit (DC) was designed. The response of TD-DC toward ONOO in vitro was assessed by luminescence intensity and lifetime. The results demonstrated that TD-DC could precisely (39 nM) detect ONOO with high selectivity and efficiency (29 s). TD-DC was further successfully employed in sensing endogenous ONOO in A-375 cells. Moreover, it could specifically localize in the mitochondrion, where endogenous ONOO is mainly generated. The above results revealed that TD-DC is efficient and useful for real-time detection of ONOO at subcellular levels.

DOI 10.1021/acs.analchem.9b03024
ISSN 1520-6882
Citation Wang Y, Li B, Song X, Shen R, Wang D, Yang Y, et al. Mito-Specific Ratiometric Terbium(III)-Complex-Based Luminescent Probe for Accurate Detection of Endogenous Peroxynitrite by Time-Resolved Luminescence Assay. Anal Chem. 2019;91(19):12422-12427.

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