Mitochondrial-targeted near-infrared fluorescence probe for selective detection of fluoride ions in living cells.

Author(s) Xu, X.; Chen, W.; Yang, M.; Liu, X.J.; Wang, F.; Yu, R.Q.; Jiang, J.H.
Journal Talanta
Date Published 2019 Nov 01
Abstract

Fluoride ions (F), as a strongest electron withdrawing anion of the smallest size, have played an indispensable role in biological systems. Here a mitochondria-targeted near-infrared (NIR) fluorescent probe (Mito-FP) is developed for selective detection of F in living cells. Mito-FP is designed using a NIR hemicyanine as a fluorophore and tert-butyldimethylsilyl (TBDMS) as a recognition site which is linked to the fluorophore via a 4-hydroxylbenzyl alcohol self-immolative linker. Mito-FP is essentially nonfluorescent. F can specifically cleave the Si-O bond which triggers the elimination of the quinone methide intermediate and generates the initial NIR hemicyanine fluorophore with distinct intramolecular charge transfer and activated fluorescence signal. The Mito-FP probe is responsive to the concentrations of F in the range of 10-120 μM with an estimated limit of detection of 3.2 μM. Mito-FP also exhibits high selectivity toward F with good stability in the physiological pH conditions. Mito-FP is successfully introduced to detect and image F in HeLa cells. Moreover, Mito-FP is also demonstrated to exhibit excellent mitochondrial-targeting ability. This NIR fluorescence probe could provide a useful tool for studying F in mitochondria.

DOI 10.1016/j.talanta.2019.06.028
ISSN 1873-3573
Citation Xu X, Chen W, Yang M, Liu X-, Wang F, Yu R-, et al. Mitochondrial-targeted near-infrared fluorescence probe for selective detection of fluoride ions in living cells. Talanta. 2019;204:655-662.

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