Molecular Study of Resistance of Staphylococcus aureus to Antiseptic Quaternary Ammonium Sulfate.

Author(s) Zaki, M.El Sayed; Bastawy, S.; Montasser, K.
Journal J Glob Antimicrob Resist
Date Published 2018 Dec 12
Abstract

OBJECTIVE: The goals of the present study were to i.determine the prevalence of qac and smr genes in clinical isolates of S.aureus from hospital acquired infections ii. Determine the susceptibility of S.aureus to QACs and antibiotics iii to correlate the presence of antiseptic resistance genes to antibiotics resistance.

METHOD: The study included 150 non replicate clinical isolates of S.aureus. The isolates were subjected to antibiotics tests by discs diffusion method and determination of susceptibility to benzalkonium chloride (BAC) by minimal inhibitory concentration method and resistant strains were subjected to study of qac and smr genes by multiplex polymerase chain reaction (PCR).

RESULTS: The reduced susceptibility to BAC was most frequent with MIC concentration 8mg/l (30%).The resistant genes to QAC were detected in 13 isolates of S. aureus with reduced susceptibility to BAC. The most frequent detected genes were qacA/B in 10 isolates (76.8%), qacJ in 10 isolates (76.8%), smr in 8 isolates (61.5%) and qacG in 8 isolates (61.5%) with low prevalence of qacH in 3 isolates (23.1%). There was strong positive correlation between the presence of QAC genes and higher MIC of BAC associated with qacA/B, qacJ and smr genes,. There was statistically significant prevalence of antiseptic resistant genes among isolates resistant to cefoxitin, ciprofloxacin, clindamycin, oxacillin, tetracyclin (P=0.0001) and erythromycin P=0.002.

CONCLUSION: The study highlights the prevalence of qac and smr genes in clinical isolates of S.aureus with resistance to quaternary ammonium sulfate. There was an association between the presence of resistant genes to antiseptic and resistance to different antibiotics which may be attributed to presence of both groups of the genes on the same plasmid or due to selection of resistant strains. There is a need to more studies about the clinical relevance of the presence of genes that control resistance to antiseptic solutions.

DOI 10.1016/j.jgar.2018.11.022
ISSN 2213-7173
Citation Zaki ME, Bastawy S, Montasser K. Molecular Study of Resistance of Staphylococcus aureus to Antiseptic Quaternary Ammonium Sulfate. J Glob Antimicrob Resist. 2018.

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