Monitoring of macrophage accumulation in statin-treated atherosclerotic mouse model using sodium iodide symporter imaging system.

Author(s) Yoo, R.Ji; Kim, M.Hwan; Woo, S.K.; Kim, K.Il; Lee, T.Sup; Choi, Y.K.; Kang, J.Hyun; Lim, S.Moo; Lee, Y.Jin
Journal Nucl Med Biol
Date Published 2017 May

INTRODUCTION: Macrophages play a key role in atherosclerotic plaque formation in atherosclerosis, but its detailed understanding has poorly investigated until now. Thus, we sought to demonstrate a noninvasive technique for macrophage tracking to atherosclerotic lesions in apolipoprotein E(-/-)(ApoE(-/-)) mice with an imaging system based on sodium iodide symporter (NIS) gene coupled with (99m)Tc-single-photon emission computed tomography (SPECT).

METHODS AND RESULTS: Macrophage cells (RAW264.7) were stably transduced with retrovirus expressing NIS gene (RAW-NIS). In RAW-NIS cells, uptake of (125)I was higher than the parental cells. [(18)F]FDG signals in the aorta at 30weeks on an ApoE(-/-) mice with high cholesterol diet were higher (1.7±0.12% injected dose (ID)) than those in control group (0.84±0.06% ID). Through (99m)Tc-SPECT/computed tomography (CT), in the RAW-NIS cell injected group, the (99m)Tc-pertechnetate uptake in aorta was higher than control groups. However, according to atorvastatin treatment, RAW-NIS cell recruitment reduced to the aorta. Area of (99m)Tc-pertechnetate uptake was positively correlated with immunostaining results against macrophage antigen (CD68). Cholesterol and low-density lipoprotein levels of atorvastatin-treated group showed lower than those of atorvastatin-untreated group, but did not reach statistical difference.

CONCLUSIONS: This novel approach to tracking macrophages to atherosclerotic plaques in vivo can be applied for studies of arterosclerotic vascular disease.

DOI 10.1016/j.nucmedbio.2017.01.009
ISSN 1872-9614
Citation Nucl Med Biol. 2017;48:4551.

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