Mortality, growth and metabolic responses by H-NMR-based metabolomics of earthworms to sodium selenite exposure in soils.

Author(s) Shao, X.; He, J.; Liang, R.; Lu, Y.; Shi, Y.; Wang, Y.; Zheng, X.; Zhang, S.; Wang, T.
Journal Ecotoxicol Environ Saf
Date Published 2019 Oct 15

The rapid development of selenium-enriched agriculture leads to the accumulation of selenium in the soil, which has an adverse impact on terrestrial ecosystems. In the present study, the mortality, growth inhibition rate and metabolism of earthworms were examined to investigate the toxicological effects of sodium selenite (NaSeO) on earthworms (Eisenia fetida) after exposuring for 14 days (d). We used H-NMR-based metabolomics to identify sensitive biomarkers and explored the metabolic responses of earthworms exposed to NaSeO. The mortality and growth inhibition rate of earthworms exposed to 70 and 90 mg/kg NaSeO were significantly higher than the rate of control group. The LC (the median lethal concentration) of NaSeO was 57.4 mg/kg in this artificial soil test of E. fetida exposed to NaSeO for 14 d. However, there was no significant differences when earthworms were exposed to different concentrations of NaSeO. The selected metabolic markers were ATP, lactic acid, leucine, alanine, valine, glycine, glutamic acid, lysine, α-glucose and betaine. NaSeO affected the metabolic level of earthworms, as the percentage of metabolic markers in the earthworm changes when exposed to different concentrations of NaSeO. The metabolic disturbances were greater with increasing concentrations of NaSeO. The differential metabolic markers were significantly changed when exposed to NaSeO comparing to those in the control group, affecting the tricarboxylic acid cycle process and breaking the metabolic balance. This study showed that NaSeO had toxic effect on the growth and development of earthworms. In addition, this study provided a biochemical insights for the development of selenium-enriched agriculture.

DOI 10.1016/j.ecoenv.2019.05.050
ISSN 1090-2414
Citation Ecotoxicol Environ Saf. 2019;181:6977.

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