Performance of a MALDI-TOF MS-based imipenem hydrolysis assay incorporating zinc sulfate.

Author(s) Knox, J.; Palombo, E.
Journal Diagn Microbiol Infect Dis
Date Published 2017 Mar
Abstract

A MALDI-TOF MS(1)-based imipenem hydrolysis assay was modified by adding ZnSO4. This improved detection of metallo-β-lactamase producing strains without compromising detection of other carbapenemase types. Using 129 genetically characterized Gram-negative bacilli, the sensitivity and specificity were 98.5% (95% confidence interval [CI]: 91.9-99.7%) and 100% (95% CI: 94.3-100%), respectively.

DOI 10.1016/j.diagmicrobio.2016.11.018
ISSN 1879-0070
Citation Knox J, Palombo E. Performance of a MALDI-TOF MS-based imipenem hydrolysis assay incorporating zinc sulfate. Diagn Microbiol Infect Dis. 2017;87(3):258-260.

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