Potentiometric detection of ATP based on the transmembrane proton gradient generated by ATPase reconstituted on a gold electrode.

Author(s) García-Molina, G.; Natale, P.; Valenzuela, L.; Alvarez-Malmagro, J.; Gutiérrez-Sánchez, C.; Iglesias-Juez, A.; López-Montero, I.; Vélez, M.; Pita, M.; De Lacey, A.L.
Journal Bioelectrochemistry
Date Published 2020 Jun
Abstract

Adenosine triphosphate (ATP) is a key molecule as energy vector for living organisms, therefore its detection reveals the presence of microbial colonies. Environments where the existence of microbial pathogens suppose a health hazard can benefit from real time monitoring of such molecule. We report a potentiometric biosensor based on ATP-synthase from Escherichia coli reconstituted in a floating phospholipid bilayer over gold electrodes modified with a 4-aminothiophenol self-assembled monolayer. The use of a pH-dependent redox probe on the electrode surface allows a simple, specific and reliable on site determination of ATP concentration from 1 μM to 1 mM. The broad range ATP biosensor can offer an alternative way of measuring in a few minutes the presence of microbial contamination.

DOI 10.1016/j.bioelechem.2020.107490
ISSN 1878-562X
Citation Bioelectrochemistry. 2020;133:107490.