Presence of nano-sized chitosan-layered silicate composites protects against toxicity induced by lead ions.

Author(s) Liu, X.; Huang, R.; Zhou, X.; Cai, T.; Chen, J.; Shi, X.; Deng, H.; Luo, W.
Journal Carbohydr Polym
Date Published 2017 Feb 20
Abstract

Protecting cells from toxicosis even apoptosis induced by a variety of toxic heavy metals stimulus has drawn more and more attentions. This study was designed to elucidate whether chitosan-organic rectorite (CS-OREC) composites exhibited any protective effects on altered oxidative stress parameter in PC12 cells exposed to lead ions (Pb(2+)). The cells were exposed to Pb(2+) either alone or in combination with CS-OREC composites for designated time to evaluate the efficacy of the composites on Pb(2+)-induced toxicity. The MTT assay results showed that the cell viability of PC12 was remarkably decreased when exposed to Pb(2+), but significantly retained after adding CS-OREC composites compared to that of the control. The beneficial effect of CS-OREC composites on cytotoxicity was related, at least in part, to its ability to protect against apoptosis in PC12 cells exposed to 50μM Pb(2+). Their protective effect was also associated with the inhibitory effect on Pb(2+)-induced activation of Bax/Bcl-2, P-38, and caspase-3 pathways, while was independent on JNK pathway.

DOI 10.1016/j.carbpol.2016.11.084
ISSN 1879-1344
Citation Liu X, Huang R, Zhou X, Cai T, Chen J, Shi X, et al. Presence of nano-sized chitosan-layered silicate composites protects against toxicity induced by lead ions. Carbohydr Polym. 2017;158:1-10.

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