Repeated-batch production of omega-3 enriched biomass of Chlorella sorokiniana via calcium-induced homeoviscous adaptation.

Author(s) Shim, S.Jin; Hong, M.Eui; Chang, W.Seok; Sim, S.Jun
Journal Bioresour Technol
Date Published 2020 May
Abstract

This study aimed to improve valuable omega-3 fatty acids production in freshwater microalgae at normal temperature by inducing homeoviscous adaptation using CaCl, which could have a role in decreasing the cellular membrane fluidity followed by increasing the rigidity of cell wall and membranes. At 10 mM CaCl, simultaneous biomass and lipid production was obtained by Ca-based single strategy without considerable sacrifice of cellular logarithmic growth in Chlorella sorokiniana. The cells cultured at 10 mM CaCl (1-stage) showed relatively high levels of cellular membrane fluidity, caused by increased content in unsaturated fatty acids, compared to the conventional culture strategy (2-stage). Moreover, when this process was recycled by repeated-batch fermentation, the EPA productivity of 1-stage was 4.338 mg L d, conspicuously increased by over 1300% compared to 2-stage. This strategy enhances the valuable omega-3 production, which can be commercially used for mass cultivation of omega-3-enriched biomass in the microalgae industry.

DOI 10.1016/j.biortech.2020.122944
Keywords Biomass; Calcium; Chlorella; Fatty Acids, Omega-3; Microalgae
ISSN 1873-2976
Citation Shim SJ, Hong ME, Chang WS, Sim SJ. Repeated-batch production of omega-3 enriched biomass of Chlorella sorokiniana via calcium-induced homeoviscous adaptation. Bioresour Technol. 2020;303:122944.