Responsive nanosensor for ratiometric luminescence detection of hydrogen sulfide in inflammatory cancer cells.

Author(s) Liu, J.; Duan, C.; Zhang, W.; Ta, H.Thu; Yuan, J.; Zhang, R.; Xu, Z.Ping
Journal Anal Chim Acta
Date Published 2020 Mar 22
Abstract

Gasotransmitter hydrogen sulfide (HS), produced enzymatically in body, has important functions in biological signaling and metabolic processes. An abnormal level of HS expression is associated with different diseases, therefore, development of novel bioanalytical methods for rapid and effective detection of HS in biological conditions is of great importance. In this work, we report the development of a new responsive nanosensor for ratiometric luminescence detection of HS in aqueous solution and live cells. The nanosensor (Ru@FITC-MSN) was prepared by immobilizing a luminescent ruthenium(II) (Ru(II)) complex into a fluorescein isothiocyanate (FITC) conjugated water-dispersible mesoporous silica nanoparticle (MSN), showing dual emission bands at 520 nm (FITC) and 600 nm (Ru complex). The red luminescence of the formed Ru@FITC-MSN was quenched in the presence of Cu. The in-situ generated Ru-Cu@FITC-MSN responded to HS rapidly and selectively, showing a linear ratiometric luminescence change in FITC and Ru(II) channels with the HS concentration (0.5-4 μM). Limit of detection (LoD) and limit of quantification (LoQ) were determined to be 0.36 and 1.21 μM. Followed by investigation of cellular uptake processes, the utility of the nanosensor for ratiometric imaging of HS in live cells and its capability to monitor HS levels in inflammatory breast cancer cells were then demonstrated. This study provides a powerful approach for detection of highly reactive and unstable HS biomolecules in live systems.

DOI 10.1016/j.aca.2019.12.056
ISSN 1873-4324
Citation Liu J, Duan C, Zhang W, Ta HT, Yuan J, Zhang R, et al. Responsive nanosensor for ratiometric luminescence detection of hydrogen sulfide in inflammatory cancer cells. Anal Chim Acta. 2020;1103:156-163.

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