Sequential Photodynamic Therapy with Phthalocyanine Encapsulated Chitosan-Tripolyphosphate Nanoparticles and Flucytosine Treatment against .

Author(s) Hsieh, Y.H.; Chuang, W.C.; Yu, K.H.; Jheng, C.P.; Lee, C.I.
Journal Pharmaceutics
Date Published 2019 Jan 04
Abstract

Antibiotic resistance has become a crisis. () is one of the most highly virulent and drug-resistant pathogens. An alternative antimicrobial therapy to eradicate effectively, without the risk of developing drug-resistance, is needed. Photodynamic therapy (PDT) is an alternative therapy that does not carry the risk of undesired drug resistance. To target the pathogens and to enhance the cellular penetration of the applied photosensitizer, we fabricated cationic chitosan/tripolyphosphate nanoparticles to encapsulate phthalocyanine. Our strategy promotes the uptake of phthalocyanine four-fold. This enhanced PDT can effectively inhibit planktonic , such that only ~20% of in the test survived; but it has a limited ability to inhibit adherent . Further tests with adherent indicated that sequential treatment with PDT and flucytosine significantly eliminates pseudohyphae and yeast-like cells. The cell viability is only ~10% after this sequential treatment. This study provides evidence of an effective therapy against drug resistant , and this strategy can be potentially applied to other pathogens.

DOI 10.3390/pharmaceutics11010016
ISSN 1999-4923
Citation Hsieh Y-, Chuang W-, Yu K-, Jheng C-, Lee C-. Sequential Photodynamic Therapy with Phthalocyanine Encapsulated Chitosan-Tripolyphosphate Nanoparticles and Flucytosine Treatment against . Pharmaceutics. 2019;11(1).

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