Sustained Release of Hydrogen Sulfide (HS) from Poly(Lactic Acid) Functionalized 4-Hydroxythiobenzamide Microparticles to Protect Against Oxidative Damage.

Author(s) Do, A.V.; Smith, R.; Tobias, P.; Carlsen, D.; Pham, E.; Bowden, N.B.; Salem, A.K.
Journal Ann Biomed Eng
Date Published 2019 May 28
Abstract

Hydrogen sulfide (HS) has emerged as a gaseous mediator capable of exhibiting many beneficial properties including cytoprotection, anti-inflammation, and vasodilation. The study presented here provides characterization of a poly(lactic acid) polymer with a functionalized 4-hydroxythiobenzamide (PLA-4HTB) capable of extended HS release. The polymer was used to fabricate microparticles that can be potentially loaded with a drug allowing for co-release of the drug and HS. Microparticles with the average diameter of 500 ± 207 nm were fabricated and shown to release 77.0 ± 1.76 µM of HS over 4 weeks (release of HS from 1 mg of particles). To test for the antioxidant properties of the PLA-4HTB microparticles, human embryonic kidney 293 cells were first incubated with PLA-4HTB microparticles and then oxidative stress was induced using CoCl. Particle suspensions of 1 mg/mL were shown to protect cells resulting in reactive oxygen species (ROS) levels of superoxide that were similar to that of the control group. The microparticles fabricated from the PLA-4HTB released HS over a sustained period of weeks to months, while providing protection from ROS. The microparticles described in this article represent a new platform technology that could be used to prevent and treat diseases caused by oxidative damage.

DOI 10.1007/s10439-019-02270-9
ISSN 1573-9686
Citation Do A-, Smith R, Tobias P, Carlsen D, Pham E, Bowden NB, et al. Sustained Release of Hydrogen Sulfide (HS) from Poly(Lactic Acid) Functionalized 4-Hydroxythiobenzamide Microparticles to Protect Against Oxidative Damage. Ann Biomed Eng. 2019.

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