Synthesis of Injectable Shear-Thinning Biomaterials of Various Compositions of Gelatin and Synthetic Silicate Nanoplatelet.

Author(s) Xue, C.; Xie, H.; Eichenbaum, J.; Chen, Y.; Wang, Y.; van den Dolder, F.W.; Lee, J.; Lee, K.J.; Zhang, S.; Sun, W.; Sheikhi, A.; Ahadian, S.; Ashammakhi, N.; Dokmeci, M.R.; Kim, H.J.; Khademhosseini, A.
Journal Biotechnol J
Date Published 2020 Feb 27
Abstract

Injectable shear-thinning biomaterials (iSTBs) have great potential for in situ tissue regeneration through minimally invasive therapeutics. Previously, an iSTB was developed by combining gelatin with synthetic silicate nanoplatelets (SNPs) for the potential application to hemostasis and endovascular embolization. Hence, we synthesize iSTBs by varying compositions of gelatin and SNPs to navigate their material, mechanical, rheological and bioactive properties. All of the compositions (each component %; 1.5 to 4.5% / total solid ranges; 3 to 9%) that we tested were injectable through both 5 Fr general catheter and 2.4 Fr microcatheter by manual pressure. In our results, an increase of gelatin contents causes the decrease of swellability, increase porosity, increase degradability and injection force during iSTB fabrication. On the other hands, the amount of SNPs in composite hydrogels are mainly required to decrease degradability and increase shear thinning properties of iSTB.Finally, in vitro and in vivo biocompatibility tests showed that the 1.5-4.5% range Gelatin-SNP iSTBs were not toxic to the cells and animals. All results demonstrated that the iSTB can be modulated with specific properties for unmet clinical needs. Understanding of mechanical and biological consequences of the changing gelatin-SNP ratios through this study will shed light on the biomedical applications of iSTB on specific diseases. This article is protected by copyright. All rights reserved.

DOI 10.1002/biot.201900456
ISSN 1860-7314
Citation Biotechnol J. 2020:e1900456.

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