Tacrolimus ameliorates the phenotypes of type 4 Bartter syndrome model mice through activation of sodium-potassium-2 chloride cotransporter and sodium-chloride cotransporter.

Author(s) Matsuura, Y.; Nomura, N.; Shoda, W.; Mori, T.; Isobe, K.; Susa, K.; Ando, F.; Sohara, E.; Rai, T.; Uchida, S.
Journal Biochem Biophys Res Commun
Date Published 2019 Sep 17
Abstract

Type 4 Bartter syndrome (BS) is caused by genetic mutations in barttin, which is coded for by BSND. Barttin serves as the β-subunit of the ClC-K chloride (Cl) channel, which is widely expressed in distal nephrons. Type 4 BS is characterized by severely impaired reabsorption of salt, which may cause polyuria, hypokalemia, and metabolic alkalosis. Calcineurin inhibitors reportedly induce renal salt retention and hyperkalemia by enhancing the phosphorylation of the sodium (Na)-potassium (K)-2Cl cotransporter (NKCC2) and Na-Cl cotransporter (NCC). In addition, we have previously reported that tacrolimus, a calcineurin inhibitor, increases the levels of phosphorylated NCC. In this study, we administered tacrolimus to barttin hypomorphic (Bsnd) mice, a murine model of type 4 BS that exhibits polyuria, hypokalemia, and metabolic alkalosis. Administration of tacrolimus increased the serum K level and suppressed urinary K excretion. Furthermore, after treatment with tacrolimus, Bsnd mice increased levels of phosphorylated NCC and NKCC2. We conclude that tacrolimus partially improves clinical phenotypes of Bsnd mice, and that calcineurin inhibitors might be effective for treating type 4 BS.

DOI 10.1016/j.bbrc.2019.07.086
ISSN 1090-2104
Citation Matsuura Y, Nomura N, Shoda W, Mori T, Isobe K, Susa K, et al. Tacrolimus ameliorates the phenotypes of type 4 Bartter syndrome model mice through activation of sodium-potassium-2 chloride cotransporter and sodium-chloride cotransporter. Biochem Biophys Res Commun. 2019;517(2):364-368.

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