Tau folding and cytotoxicity of neuroblastoma cells in the presence of manganese oxide nanoparticles: Biophysical, molecular dynamics, cellular, and molecular studies.

Author(s) Mehdizadeh, P.; Fesharaki, S.Sadat Hash; Nouri, M.; Ale-Ebrahim, M.; Akhtari, K.; Shahpasand, K.; Saboury, A.Akbar; Falahati, M.
Journal Int J Biol Macromol
Date Published 2019 Mar 15

Manganese oxide nanoparticles (MnO NPs) have been widely used in the medical and biological applications. However, few studies have been undertaken to investigate the cytotoxicity of MnO NPs against nervous system. Herein, we studied the toxicity of MnO NPs against tau protein and neuroblastoma cells (SH-SY5Y) in vitro. Circular dichroism (CD) spectroscopy, fluorescence spectroscopy, molecular docking, and molecular dynamic studies were used to explore the conformational changes of protein. The cell-based experiments, such as viability, activation of caspases-3/9, apoptosis, and gene (Bax and Bcl-2) expression assays were performed in vitro. Spectroscopic methods and molecular dynamic studies revealed that MnO NPs can fold the structure of tau toward a more packed structure. The MnO NPs also decreased the cell viability in a dose-dependent manner. Indeed, caspase-3 and caspase-9 activation, Bax/Bcl-2 ratio elevation and apoptosis induction were observed after exposure of SH-SY5Y to MnO NPs. In conclusion, tau folding and cytotoxicity against SH-SY5Y cells may be involved in adverse effects induced by MnO NPs.

DOI 10.1016/j.ijbiomac.2018.11.191
ISSN 1879-0003
Citation Int J Biol Macromol. 2019;125:674682.

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