Three-Dimensional Cadmium Telluride Quantum Dots-DNA Nanoreticulation as a Highly Efficient Electrochemiluminescent Emitter for Ultrasensitive Detection of MicroRNA from Cancer Cells.

Author(s) Sun, M.F.; Liu, J.L.; Chai, Y.Q.; Zhang, J.; Tang, Y.; Yuan, R.
Journal Anal Chem
Date Published 2019 Jun 18

In this work, a novel three-dimensional cadmium telluride quantum dots-DNA nanoreticulation (3D CdTe QDs-DNA-NR) was used as a signal probe with the dual-legged DNA walker circular amplification as target conversion strategy to establish a pioneering electrochemiluminescence (ECL) biosensing strategy for ultrasensitive detection of microRNA-21 form cancer cells. Herein, such a 3D luminous nanomaterial with reticular structure not only supported abundant CdTe QDs to avoid the inner filter effect for obtaining a high ECL efficiency but also contained the hemin/G-quadruplex as coreaction accelerator in the 3D CdTe QDs-DNA-NR/SO system for the enhancement of ECL intensity. Furthermore, with the target-induced dual-legged DNA walker circular amplification strategy, a mass of output DNA was produced to connect with the 3D CdTe QDs-DNA-NR for the construction of the ECL biosensor, which realized the ultrasensitive detection of microRNA-21 from 100 aM to 100 pM and the detection limit down to 34 aM. Significantly, this work could be readily extended for the detection of other biomolecules to provide a neoteric channel for disease diagnosis.

DOI 10.1021/acs.analchem.9b01185
ISSN 1520-6882
Citation Anal Chem. 2019;91(12):77657773.

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