Very low concentration of cerium dioxide nanoparticles induce DNA damage, but no loss of vitality, in human spermatozoa.

Author(s) Préaubert, L.; Tassistro, V.; Auffan, M.; Sari-Minodier, I.; Rose, J.; Courbiere, B.; Perrin, J.
Journal Toxicol In Vitro
Date Published 2018 Apr 04
Abstract

Cerium dioxide nanoparticles (CeONP) are widely used for industrial purposes, as in diesel, paint, wood stain and as potential therapeutic applications. The Organization for Economic Cooperation and Development included CeONP in the priority list of nanomaterials requiring urgent evaluation. As metal nanoparticles can cross the blood-testis barrier, CeONP could interact with spermatozoa. The genotoxicity of CeONP was demonstrated in vitro on human cell lines and mouse gametes. However, the effects of CeONP on human spermatozoa DNA remain unknown. We showed significant DNA damage induced in vitro by CeONP on human spermatozoa using Comet assay. The genotoxicity was inversely proportional to the concentration (0.01 to 10 mg·L). TEM showed no internalization of CeONP into the spermatozoa. This study shows for the first time that in vitro exposure to very low concentrations of cerium dioxide nanoparticles can induce significant DNA damage in human spermatozoa. These results add new and important insights regarding the reproductive toxicity of priority nanomaterials, which require urgent evaluation.

DOI 10.1016/j.tiv.2018.03.013
ISSN 1879-3177
Citation Préaubert L, Tassistro V, Auffan M, Sari-Minodier I, Rose J, Courbiere B, et al. Very low concentration of cerium dioxide nanoparticles induce DNA damage, but no loss of vitality, in human spermatozoa. Toxicol In Vitro. 2018;50:236-241.

Related Applications, Forms & Industries