Zn(2+) reduction induces neuronal death with changes in voltage-gated potassium and sodium channel currents.

Title Zn(2+) reduction induces neuronal death with changes in voltage-gated potassium and sodium channel currents.
Authors K. Tian; C.C. He; H.N. Xu; Y.X. Wang; H.G. Wang; D. An; B. Heng; W. Pang; Y.G. Jiang; Y.Q. Liu
Journal J Trace Elem Med Biol
DOI 10.1016/j.jtemb.2017.02.011
Abstract

In the present study, cultured rat primary neurons were exposed to a medium containing N,N,N',N'-tetrakis(2-pyridylmethyl)ethylenediamine (TPEN), a specific cell membrane-permeant Zn(2+) chelator, to establish a model of free Zn(2+) deficiency in neurons. The effects of TPEN-mediated free Zn(2+) ion reduction on neuronal viability and on the performance of voltage-gated sodium channels (VGSCs) and potassium channels (Kvs) were assessed. Free Zn(2+) deficiency 1) markedly reduced the neuronal survival rate, 2) reduced the peak amplitude of INa, 3) shifted the INa activation curve towards depolarization, 4) modulated the sensitivity of sodium channel voltage-dependent inactivation to a depolarization voltage, and 5) increased the time course of recovery from sodium channel inactivation. In addition, free Zn(2+) deficiency by TPEN notably enhanced the peak amplitude of transient outward K(+) currents (IA) and delayed rectifier K(+) currents (IK), as well as caused hyperpolarization and depolarization directional shifts in their steady-state activation curves, respectively. Zn(2+) supplementation reversed the effects induced by TPEN. Our results indicate that free Zn(2+) deficiency causes neuronal damage and alters the dynamic characteristics of VGSC and Kv currents. Thus, neuronal injury caused by free Zn(2+) deficiency may correlate with its modulation of the electrophysiological properties of VGSCs and Kvs.

Citation K. Tian; C.C. He; H.N. Xu; Y.X. Wang; H.G. Wang; D. An; B. Heng; W. Pang; Y.G. Jiang; Y.Q. Liu.Zn(2+) reduction induces neuronal death with changes in voltage-gated potassium and sodium channel currents.. J Trace Elem Med Biol. 2017;41:6674. doi:10.1016/j.jtemb.2017.02.011

Related Elements

Potassium

Elemental PotassiumSee more Potassium products. Potassium (atomic symbol: K, atomic number: 19) is a Block S, Group 1, Period 4 element with an atomic weight of 39.0983. The number of electrons in each of Potassium's shells is [2, 8, 8, 1] and its electron configuration is [Ar] 4s1. The potassium atom has a radius of 227.2 pm and a Van der Waals radius of 275 pm. Potassium was discovered and first isolated by Sir Humphrey Davy in 1807. Potassium is the seventh most abundant element on earth. It is one of the most reactive and electropositive of all metals and rapidly oxidizes. As with other alkali metals, potassium decomposes in water with the evolution of hydrogen because of its reacts violently with water, it only occurs in nature in ionic salts.Potassium Bohr Model In its elemental form, potassium has a silvery gray metallic appearance, but its compounds (such as potassium hydroxide) are more frequently used in industrial and chemical applications. The origin of the element's name comes from the English word 'potash,' meaning pot ashes, and the Arabic word qali, which means alkali. The symbol K originates from the Latin word kalium.

Sodium

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Zinc

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