Synergistic effect of strontium and silicon in strontium-substituted sub-micron bioactive glass for enhanced osteogenesis.

Title Synergistic effect of strontium and silicon in strontium-substituted sub-micron bioactive glass for enhanced osteogenesis.
Authors W. Zhang; D. Huang; F. Zhao; W. Gao; L. Sun; X. Li; X. Chen
Journal Mater Sci Eng C Mater Biol Appl
DOI 10.1016/j.msec.2018.04.012
Abstract

Strontium-substituted sub-micron bioactive glasses (Sr-SBG) have been reported to have enhanced osteogenic differentiation capacity compared to sub-micron bioactive glasses (SBG) in our previous study. However, the underlying molecular mechanisms of such beneficial effect of Sr-SBG are still not fully understood. In this study, we synthesized Sr-SBG, studied the effects of Sr-SBG on proliferation and osteogenic differentiation of mouse mesenchymal stem cells (mMSCs), and identified the molecular mechanisms of the enhancement effect of Sr-SBG on mMSCs. The results demonstrated that Sr-SBG had more profound promotion effect on proliferation and osteogenic differentiation of mMSCs than SBG and SrCl group which containing identical Sr concentration with Sr-SBG group. RT-qPCR and western blot analysis showed that the mRNA expressions and protein expressions involved in NFATc and Wnt/?-catenin signaling pathways were all upregulated mediated by Sr-SBG, while only Wnt/?-catenin signaling pathway related genes upregulated in SBG group and only NFATc signaling pathway activated in SrCl group, suggesting that NFATc and Wnt/?-catenin signaling pathways played important roles in osteogenesis enhancement induced by Sr-SBG. To conform the above conclusion, cyclosporin A (CSA) was applied to inhibit NFATc signaling pathway. It was found that the enhanced osteogenic differentiation of mMSCs induced by Sr-SBG was partially abrogated and the activated Wnt/?-catenin signaling pathway was also inhibited in part. However, the effects of SBG on proliferation and osteogenesis of mMSCs were unimpaired, yet the effects of SrCl were greatly suppressed. Taken together, these results indicated that strontium activated NFATc signaling pathway and silicate activated Wnt/?-catenin signaling pathway might synergistically mediated the enhanced osteogenesis induced by Sr-SBG.

Citation W. Zhang; D. Huang; F. Zhao; W. Gao; L. Sun; X. Li; X. Chen.Synergistic effect of strontium and silicon in strontium-substituted sub-micron bioactive glass for enhanced osteogenesis.. Mater Sci Eng C Mater Biol Appl. 2018;89:245255. doi:10.1016/j.msec.2018.04.012

Related Elements

Strontium

See more Strontium products. Strontium (atomic symbol: Sr, atomic number: 38) is a Block S, Group 2, Period 5 element with an atomic weight of 87.62 . Strontium Bohr ModelThe number of electrons in each of Strontium's shells is [2, 8, 18, 8, 2] and its electron configuration is [Kr] 5s2. The strontium atom has a radius of 215 pm and a Van der Waals radius of 249 pm. Strontium was discovered by William Cruickshank in 1787 and first isolated by Humphry Davy in 1808. In its elemental form, strontium is a soft, silvery white metallic solid that quickly turns yellow when exposed to air. Elemental StrontiumCathode ray tubes in televisions are made of strontium, which are becoming increasingly displaced by other display technologies pyrotechnics and fireworks employ strontium salts to achieve a bright red color. Radioactive isotopes of strontium have been used in radioisotope thermoelectric generators (RTGs) and for certain cancer treatments. In nature, most strontium is found in celestite (as strontium sulfate) and strontianite (as strontium carbonate). Strontium was named after the Scottish town where it was discovered.

Silicon

See more Silicon products. Silicon (atomic symbol: Si, atomic number: 14) is a Block P, Group 14, Period 3 element with an atomic weight of 28.085. Silicon Bohr MoleculeThe number of electrons in each of Silicon's shells is 2, 8, 4 and its electron configuration is [Ne] 3s2 3p2. The silicon atom has a radius of 111 pm and a Van der Waals radius of 210 pm. Silicon was discovered and first isolated by Jöns Jacob Berzelius in 1823. Silicon makes up 25.7% of the earth's crust, by weight, and is the second most abundant element, exceeded only by oxygen. The metalloid is rarely found in pure crystal form and is usually produced from the iron-silicon alloy ferrosilicon. Elemental SiliconSilica (or silicon dioxide), as sand, is a principal ingredient of glass, one of the most inexpensive of materials with excellent mechanical, optical, thermal, and electrical properties. Ultra high purity silicon can be doped with boron, gallium, phosphorus, or arsenic to produce silicon for use in transistors, solar cells, rectifiers, and other solid-state devices which are used extensively in the electronics industry.The name Silicon originates from the Latin word silex which means flint or hard stone.