A continuous luminescence assay for monitoring kinase activity: signalling the ADP/ATP ratio using a discrete europium complex.

Title A continuous luminescence assay for monitoring kinase activity: signalling the ADP/ATP ratio using a discrete europium complex.
Authors S.H. Hewitt; J. Parris; R. Mailhot; S.J. Butler
Journal Chem Commun (Camb)
DOI 10.1039/c7cc05887j
Abstract

We report the application of a stable cationic europium complex [Eu.1]+ in a continuous-read luminescence assay for kinase activity. [Eu.1]+ binds reversibly to ATP and ADP in water, at neutral pH, in the presence of Mg2+ ions, providing distinctive luminescence responses that permits the kinase-catalysed conversion of ATP to ADP to be monitored in real-time.

Citation S.H. Hewitt; J. Parris; R. Mailhot; S.J. Butler.A continuous luminescence assay for monitoring kinase activity: signalling the ADP/ATP ratio using a discrete europium complex.. Chem Commun (Camb). 2017;53(94):1262612629. doi:10.1039/c7cc05887j

Related Elements

Europium

See more Europium products. Europium (atomic symbol: Eu, atomic number: 63) is a Block F, Group 3, Period 6 element with an atomic radius of 151.964. Europium Bohr ModelThe number of electrons in each of Europium's shells is 2, 8, 18, 25, 8, 2 and its electron configuration is [Xe]4f7 6s2. The europium atom has an atomic radius of 180 pm and a Van der Waals radius of 233 pm. Europium was discovered by Eugène-Anatole Demarçay in 1896, however, he did not isolate it until 1901. Europium was named after the continent of Europe.Elemental Europium Picture Europium is a member of the lanthanide or rare earth series of metals. In its elemental form, it has a silvery-white appearance but it is rarely found without oxide discoloration. Europium is found in many minerals including bastnasite, monazite, xenotime and loparite. It is not found in nature as a free element.

Related Forms & Applications