Integrated amplified aptasensor with in-situ precise preparation of copper nanoclusters for ultrasensitive electrochemical detection of microRNA 21.

Title Integrated amplified aptasensor with in-situ precise preparation of copper nanoclusters for ultrasensitive electrochemical detection of microRNA 21.
Authors Y. Wang; X. Zhang; L. Zhao; T. Bao; W. Wen; X. Zhang; S. Wang
Journal Biosens Bioelectron
DOI 10.1016/j.bios.2017.07.009
Abstract

MicroRNA 21 (MIR21) has garnered much attention in recent years as an important disease biomarker. The detection of it in human system shows great significance for the healthy evaluation and major diseases early detection. Herein, a novel approach tactfully manipulates the in-situ precise preparation of copper nanoclusters on overlapping Y-shaped ds-DNA for MIR21 analysis were developed in the proposed integrated aptasensor. In the presence of target MIR21, overlapping Y-shaped ds-DNA was constructed on electrode. Copper nanoclusters were in-situ prepared on this effective template for target detection. Taking advantage of exonuclease T7 triggered targets recycling, hybridization chain reaction (HCR) and copper nanoclusters triple amplification strategy, linear detection of MIR21 was achieved from 10pM to 0.1fM with a detection limit down to 10aM (S/N > 3). This approach provides a good model for integrating both synthesis and detection into one electrochemistry component. It showed promising potential for applications in aptamer related target detection in human serum analysis.

Citation Y. Wang; X. Zhang; L. Zhao; T. Bao; W. Wen; X. Zhang; S. Wang.Integrated amplified aptasensor with in-situ precise preparation of copper nanoclusters for ultrasensitive electrochemical detection of microRNA 21.. Biosens Bioelectron. 2017;98:386391. doi:10.1016/j.bios.2017.07.009

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Copper

See more Copper products. Copper Bohr Model Copper (atomic symbol: Cu, atomic number: 29) is a Block D, Group 11, Period 4 element with an atomic weight of 63.546. The number of electrons in each of copper's shells is 2, 8, 18, 1 and its electron configuration is [Ar]3d10 4s1. The copper atom has a radius of 128 pm and a Van der Waals radius of 186 pm. Copper was first discovered by Early Man prior to 9000 BC. In its elemental form, copper has a reddish-orange metallic and lustrous appearance. Of all pure metals, only silver Elemental Copperhas a higher electrical conductivity. The origin of the word copper comes from the Latin word 'cuprium' which translates as "metal of Cyprus," as the Mediterranean island of Cyprus was known as an ancient source of mined copper..

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