"Off-On"switching electrochemiluminescence biosensor for mercury(II) detection based on molecular recognition technology.

Title "Off-On"switching electrochemiluminescence biosensor for mercury(II) detection based on molecular recognition technology.
Authors L. Cheng; B.G. Wei; L.Ling He; L. Mao; J. Zhang; J.X. Ceng; D.R. Kong; C.D. Chen; H.F. Cui; N. Hong; H. Fan
Journal Anal Biochem
DOI 10.1016/j.ab.2016.09.018
Abstract

A novel "off-On" electrogenerated chemiluminescence (ECL) biosensor has been developed for the detection of mercury(II) based on molecular recognition technology. The ECL mercury(II) biosensor comprises two main parts: an ECL substrate and an ECL intensity switch. The ECL substrate was made by modifying the complex of Ruthenium(II) tris-(bipyridine)(Ru(bpy)3(2+))/Cyclodextrins-Au nanoparticles(CD-AuNps)/Nafion on the surface of glass carbon electrode (GCE), and the ECL intensity switch is the single hairpin DNA probe designed according to the "molecular recognition" strategy which was functionalized with ferrocene tag at one end and attached to Cyclodextrins (CD) on modified GCE through supramolecular noncovalent interaction. We demonstrated that, in the absence of Hg(II) ion, the probe keeps single hairpin structure and resulted in a quenching of ECL of Ru(bpy)3(2+). Whereas, in the presence of Hg(II) ion, the probe prefers to form the T-Hg(II)-T complex and lead to an obvious recovery of ECL of Ru(bpy)3(2+), which provided a sensing platform for the detection of Hg(II) ion. Using this sensing platform, a simple, rapid and selective "off-On" ECL biosensor for the detection of mercury(II) with a detection limit of 0.1 nM has been developed.

Citation L. Cheng; B.G. Wei; L.Ling He; L. Mao; J. Zhang; J.X. Ceng; D.R. Kong; C.D. Chen; H.F. Cui; N. Hong; H. Fan."Off-On"switching electrochemiluminescence biosensor for mercury(II) detection based on molecular recognition technology.. Anal Biochem. 2017;518:4652. doi:10.1016/j.ab.2016.09.018

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Mercury

Mercury Bohr ModelSee more Mercury products. Mercury (atomic symbol: Hg, atomic number: 80) is a Block D, Group 12, Period 6 element with an atomic weight of 200.59. The number of electrons in each of mercury's shells is 2, 8, 18,32, 18, 2 and its electron configuration is [Xe] 4f14 5d10 6s2. The mercury atom has a radius of 151 pm and a Van der Waals radius of 209 pm. It is named after the planet Mercury and often referred to as "quicksilver" due to its appearance as a silvery liquid. Mercury has low melting and boiling points. It is a poor conductor of heat, but a fair conductor of electricity. Mercury is found both as a free element and in cinnabar, corderoite, and livingstonite ores.