Reversed phase ion-pair chromatographic separation of sugar alcohols by complexation with molybdate ion.

Title Reversed phase ion-pair chromatographic separation of sugar alcohols by complexation with molybdate ion.
Authors T. Kemmei; S. Kodama; A. Yamamoto; Y. Inoue; K. Hayakawa
Journal J Chromatogr A
DOI 10.1016/j.chroma.2018.03.016
Abstract

In this study, we developed a simple and sensitive reversed phase ion-pair chromatographic method for the analysis of C4-C6 sugar alcohols. The method is based on the on-line complexation of sugar alcohols with molybdate ion. The resulting dinuclear anionic complexes can be separated on a reversed-phase C18 column using tetrabutylammonium chloride as an ion-pairing reagent. The mobile phase (pH 3.1) consisted of 0.1?mM disodium molybdate, 1?mM hydrochloric acid and 0.4?mM tetrabutylammonium chloride - 10% v/v methanol. By complexing with molybdate ion, sugar alcohols can be detected by their UV absorption at 247?nm with high resolution and sensitivity. The quantification limits of the examined sugar alcohols calculated at S/N?=?10 were 0.1?mM for erythritol and xylitol and 0.01?mM for arabitol, sorbitol, mannitol and dulcitol. The detector response was linear over three orders of magnitude of sugar alcohol concentration. The proposed method was successfully applied to measure sugar alcohols in health drinks, eyedrops and mouthwashes.

Citation T. Kemmei; S. Kodama; A. Yamamoto; Y. Inoue; K. Hayakawa.Reversed phase ion-pair chromatographic separation of sugar alcohols by complexation with molybdate ion.. J Chromatogr A. 2018;1547:7176. doi:10.1016/j.chroma.2018.03.016

Related Elements

Molybdenum

See more Molybdenum products. Molybdenum (atomic symbol: Mo, atomic number: 42) is a Block D, Group 6, Period 5 element with an atomic weight of 95.96. Molybdenum Bohr ModelThe number of electrons in each of molybdenum's shells is [2, 8, 18, 13, 1] and its electron configuration is [Kr] 4d5 5s1. The molybdenum atom has a radius of 139 pm and a Van der Waals radius of 209 pm. In its elemental form, molybdenum has a gray metallic appearance. Molybdenum was discovered by Carl Wilhelm in 1778 and first isolated by Peter Jacob Hjelm in 1781. Molybdenum is the 54th most abundant element in the earth's crust. Elemental MolybdenumIt has the third highest melting point of any element, exceeded only by tungsten and tantalum. Molybdenum does not occur naturally as a free metal, it is found in various oxidation states in minerals. The primary commercial source of molybdenum is molybdenite, although it is also recovered as a byproduct of copper and tungsten mining. The origin of the name Molybdenum comes from the Greek word molubdos meaning lead.

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