Gold nanorod embedded novel 3D graphene nanocomposite for selective bio-capture in rapid detection of Mycobacterium tuberculosis.

Title Gold nanorod embedded novel 3D graphene nanocomposite for selective bio-capture in rapid detection of Mycobacterium tuberculosis.
Authors V. Perumal; M.Shuaib Moh Saheed; N.Muti Mohamed; M.Salleh Moh Saheed; S.Sundera Murthe; S.C.B. Gopinath; J.M. Chiu
Journal Biosens Bioelectron
DOI 10.1016/j.bios.2018.05.042
Abstract

Tuberculosis (TB) is a chronic and infectious airborne disease which requires a diagnosing system with high sensitivity and specificity. However, the traditional gold standard method for TB detection remains unreliable with low specificity and sensitivity. Nanostructured composite materials coupled with impedimetric sensing utilised in this study offered a feasible solution. Herein, novel gold (Au) nanorods were synthesized on 3D graphene grown by chemical vapour deposition. The irregularly spaced and rippled morphology of 3D graphene provided a path for Au nanoparticles to self-assemble and form rod-like structures on the surface of the 3D graphene. The formation of Au nanorods were showcased through scanning electron microscopy which revealed the evolution of Au nanoparticle into Au islets. Eventually, it formed nanorods possessing lengths of ~?150?nm and diameters of ~?30?nm. The X-ray diffractogram displayed appropriate peaks suitable to defect-free and high crystalline graphene with face centered cubic Au. The strong optical interrelation between Au nanorod and 3D graphene was elucidated by Raman spectroscopy analysis. Furthermore, the anchored Au nanorods on 3D graphene nanocomposite enables feasible bio-capturing on the exposed Au surface on defect free graphene. The impedimetric sensing of DNA sequence from TB on 3D graphene/Au nanocomposite revealed a remarkable wide detection linear range from 10?fM to 0.1?µM, displays the capability of detecting femtomolar DNA concentration. Overall, the novel 3D graphene/Au nanocomposite demonstrated here offers high-performance bio-sensing and opens a new avenue for TB detection.

Citation V. Perumal; M.Shuaib Moh Saheed; N.Muti Mohamed; M.Salleh Moh Saheed; S.Sundera Murthe; S.C.B. Gopinath; J.M. Chiu.Gold nanorod embedded novel 3D graphene nanocomposite for selective bio-capture in rapid detection of Mycobacterium tuberculosis.. Biosens Bioelectron. 2018;116:116122. doi:10.1016/j.bios.2018.05.042

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Gold

See more Gold products. Gold (atomic symbol: Au, atomic number: 79) is a Block D, Group 11, Period 6 element with an atomic weight of 196.966569. The number of electrons in each of Gold's shells is 2, 8, 18, 32, 18, 1 and its electron configuration is [Xe]4f142 5d10 6s1. Gold Bohr ModelThe gold atom has a radius of 144 pm and a Van der Waals radius of 217 pm. Gold was first discovered by Early Man prior to 6000 B.C. In its elemental form, gold has a metallic yellow appearance. Gold is a soft metal and is usually alloyed to give it more strength.Elemental Gold It is a good conductor of heat and electricity, and is unaffected by air and most reagents. It is one of the least reactive chemical elements. Gold is often found as a free element and with silver as a gold-silver alloy. Less commonly, it is found in minerals as gold compounds, usually with tellurium.

Carbon

See more Carbon products. Carbon (atomic symbol: C, atomic number: 6) is a Block P, Group 14, Period 2 element. Carbon Bohr ModelThe number of electrons in each of Carbon's shells is 2, 4 and its electron configuration is [He]2s2 2p2. In its elemental form, carbon can take various physical forms (known as allotropes) based on the type of bonds between carbon atoms; the most well known allotropes are diamond, graphite, amorphous carbon, glassy carbon, and nanostructured forms such as carbon nanotubes, fullerenes, and nanofibers . Carbon is at the same time one of the softest (as graphite) and hardest (as diamond) materials found in nature. It is the 15th most abundant element in the Earth's crust, and the fourth most abundant element (by mass) in the universe after hydrogen, helium, and oxygen. Carbon was discovered by the Egyptians and Sumerians circa 3750 BC. It was first recognized as an element by Antoine Lavoisier in 1789.

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