Determination of prostate cancer biomarker acid phosphatase at a copper phthalocyanine-modified screen printed gold transducer.

Author(s) Fredj, Z.; Ben Ali, M.; Abbas, M.Nooredeen; Dempsey, E.
Journal Anal Chim Acta
Date Published 2019 May 30

In this work, a novel sensor based on immobilised copper phthalocyanine, 2,9,16,23-tetracarboxylic acid-polyacrylamide (Cu(II)TC Pc-PAA) was developed for determination of acid phosphatase (ACP) levels in nanomolar quantities. Detection was based on the measurement of enzymatically generated phosphate, with initial studies focused on phosphate detection at a Cu(II)TC Pc-PAA modified screen-printed gold transducer. The sensor was characterised in relation to operational performance (pH, response time, stability, linearity, and sensitivity) and common anionic interferents (nitrate, sulphate, chloride, and perchlorate). The functionalised surface also facilitated rapid detection of the enzyme bi-product 2-naphthol over the range 5-3000 μM. Quantitation of ACP was demonstrated, realising a linear response range of 0.5-20 nM and LOD of 0.5 nM, which is within the clinical range for this prostate cancer biomarker.

DOI 10.1016/j.aca.2018.12.058
Keywords Acid Phosphatase; Biomarkers, Tumor; Electrochemistry; Electrodes; Gold; Humans; Hydrogen-Ion Concentration; Indoles; Limit of Detection; Male; Organometallic Compounds; Perchlorates; Printing; Prostatic Neoplasms; Surface Properties; Time Factors; Transducers
ISSN 1873-4324
Citation Anal Chim Acta. 2019;1057:98105.

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