Functional Titanium Carbide MXenes-Loaded Entropy-Driven RNA Explorer for Long Noncoding RNA PCA3 Imaging in Live Cells.

Author(s) Wang, S.; Song, W.; Wei, S.; Zeng, S.; Yang, S.; Lei, C.; Huang, Y.; Nie, Z.; Yao, S.
Journal Anal Chem
Date Published 2019 May 30

The visualization of the long noncoding RNA of prostate cancer gene 3 (lncRNA PCA3), a specific biomarker for androgen receptor-positive prostate cancer, in living cells not only directly reflects the gene expression and localization, but also offers a better insight into its roles in the pathological processes. Here, we loaded an entropy-driven RNA explorer (EDRE) on the TAT peptide-functionalized titanium carbide MXenes (Ti3C2-TAT) for the imaging of nuclear lncRNA PCA3 in live cells. The EDRE was condensed on the Ti3C2-TAT (Ti3C2-TAT@EDRE) by electrostatic interaction. Ti3C2-TAT@EDRE enables to enter cells and release TAT peptides and EDRE in the cytoplasm by the glutathione (GSH)-triggered clevage of the double bonds in Ti3C2-TAT. The released EDRE is delivered into the nucleus by the nucleus-targeted guidance of TAT peptides, and initiated by the target lncRNA PCA3, subsequently leading to the continuous accumulation of fluorescence signals. As a consequence, fluorescence analysis of lncRNA PCA3 at low-picomolar concentrations in vitro, as well as sensitive live cell imaging of lncRNA PCA3 in the nuclear of androgen receptor-positive LNCaP prostate cancer cells was achieved, providing a versatile strategy for the monitoring of nucleic acid biomarkers in the nucleus of living cells.

DOI 10.1021/acs.analchem.9b02040
ISSN 1520-6882
Citation Anal Chem. 2019.

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