Impact of calcium aluminate cement with additives on dental pulp-derived cells.

Author(s) Messias, N.Saab; Grisote, G.; Martorano, A.Secco; Fernandes, R.Rodrigo; de Oliveira, I.Regina; Bombonato-Prado, K.Fittipaldi; de Oliveira, P.Tambasco; de Castro-Raucci, L.Moreira Sp
Journal J Appl Oral Sci
Date Published 2020

Calcium aluminate cement (CAC) has been highlighted as a promising alternative for endodontic use aiming at periapical tissue repair. However, its effects on dental pulp cells have been poorly explored.

OBJECTIVE: This study assessed the impact of calcium chloride (CaCl2) and bismuth oxide (Bi2O3) or zinc oxide (ZnO) additives on odontoblast cell response to CAC.

METHODOLOGY: MDPC-23 cells were exposed for up to 14 d: 1) CAC with 2.8% CaCl2 and 25% ZnO (CACz); 2) CAC with 2.8% CaCl2 and 25% Bi2O3 (CACb); 3) CAC with 10% CaCl2 and 25% Bi2O3 (CACb+); or 4) mineral trioxide aggregate (MTA), placed on inserts. Non-exposed cultures served as control. Cell morphology, cell viability, gene expression of alkaline phosphatase (ALP), bone sialoprotein (BSP), and dentin matrix protein 1 (DMP-1), ALP activity, and extracellular matrix mineralization were evaluated. Data were compared using ANOVA (α=5%).

RESULTS: Lower cell density was detected only for MTA and CACb+ compared with Control, with areas showing reduced cell spreading. Cell viability was similar among groups at days one and three (p>0.05). CACb+ and MTA showed the lowest cell viability values at day seven (p>0.05). CACb and CACb+ promoted higher ALP and BSP expression compared with CACz (p<0.05); despite that, all cements supported ALP activity. Matrix mineralization were enhanced in CACb+ and MTA.

CONCLUSION: In conclusion, CAC with Bi2O3, but not with ZnO, supported the expression of odontoblastic phenotype, but only the composition with 10% CaCl2 promoted mineralized matrix formation, rendering it suitable for dentin-pulp complex repair.

DOI 10.1590/1678-7757-2019-0105
Keywords Alkaline Phosphatase; Aluminum Compounds; Animals; Bismuth; Calcium Chloride; Calcium Compounds; Cell Survival; Cells, Cultured; Dental Cements; Dental Pulp; Drug Combinations; Gene Expression; Materials Testing; Mice; Odontoblasts; Oxides; Reproducibility of Results; Silicates; Time Factors; Zinc Oxide
ISSN 1678-7765
Citation J Appl Oral Sci. 2020;28:e20190105.

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