Magnesium Regulates the Circadian Oscillator in Cyanobacteria.

Author(s) Jeong, Y.M.; Dias, C.; Diekman, C.; Brochon, H.; Kim, P.; Kaur, M.; Kim, Y.S.; Jang, H.I.; Kim, Y.I.
Journal J Biol Rhythms
Date Published 2019 Aug
Abstract

The circadian clock controls 24-h biological rhythms in our body, influencing many time-related activities such as sleep and wake. The simplest circadian clock is found in cyanobacteria, with the proteins KaiA, KaiB, and KaiC generating a self-sustained circadian oscillation of KaiC phosphorylation and dephosphorylation. KaiA activates KaiC phosphorylation by binding the A-loop of KaiC, while KaiB attenuates the phosphorylation by sequestering KaiA from the A-loop. Structural analysis revealed that magnesium regulates the phosphorylation and dephosphorylation of KaiC by dissociating from and associating with catalytic Glu residues that activate phosphorylation and dephosphorylation, respectively. High magnesium causes KaiC to dephosphorylate, whereas low magnesium causes KaiC to phosphorylate. KaiC alone behaves as an hourglass timekeeper when the magnesium concentration is alternated between low and high levels in vitro. We suggest that a magnesium-based hourglass timekeeping system may have been used by ancient cyanobacteria before magnesium homeostasis was established.

DOI 10.1177/0748730419851655
ISSN 1552-4531
Citation Jeong YM, Dias C, Diekman C, Brochon H, Kim P, Kaur M, et al. Magnesium Regulates the Circadian Oscillator in Cyanobacteria. J Biol Rhythms. 2019;34(4):380-390.