Mitochondrial dysfunction, oxidative stress and apoptotic induction in microglial BV-2 cells treated with sodium arsenate.

Author(s) Kharroubi, W.; Ahmed, S.Haj; Nury, T.; Andreoletti, P.; Sakly, R.; Hammami, M.; Lizard, G.
Journal J Environ Sci (China)
Date Published 2017 Jan
Abstract

The treatment of microglial BV-2 cells with sodium arsenate (As(V): 0.1-400μmol/L - 48hr) induces a dose-dependent response. The neurotoxic effects of high concentrations of As(V) (100, 200 and 400μmol/L) are characterized by increased levels of mitochondrial complexes I, II, and IV followed by increased superoxide anion generation. Moreover, As(V) triggers an apoptotic mode of cell death, demonstrated by an apoptotic SubG1 peak, associated with an alteration of plasma membrane integrity. There is also a decrease in transmembrane mitochondrial potential and mitochondrial adenosine triphosphate ATP. It is therefore tempting to speculate that As(V) triggers mitochondrial dysfunction, which may lead to defective oxidative phosphorylation subsequently causing mitochondrial oxidative damage, which in turn induces an apoptotic mode of cell death.

DOI 10.1016/j.jes.2016.08.028
Keywords Adenosine Triphosphate; Animals; Apoptosis; Arsenates; Cell Line; Hazardous Substances; Membrane Potential, Mitochondrial; Mice; Mitochondria; Oxidation-Reduction; Oxidative Stress
ISSN 1001-0742
Citation J Environ Sci (China). 2017;51:4451.

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