Optimization of the photodynamic inactivation of prions by a phthalocyanine photosensitizer: the crucial involvement of singlet oxygen.

Author(s) Kostelanska, M.; Freisleben, J.; Hanusova, Z.Backovska; Mosko, T.; Vik, R.; Moravcova, D.; Hamacek, A.; Mosinger, J.; Holada, K.
Journal J Biophotonics
Date Published 2019 Apr 15
Abstract

Prion disorders are fatal neurodegenerative diseases caused by the autocatalytic conversion of a natively occurring prion protein (PrP ) into its misfolded infectious form (PrP ). The proven resistance of PrP to common disinfection procedures increases the risk of prion transmission in medical settings. Herein, we present the effective photodynamic inactivation (PDI) of prions by disulfonated hydroxyaluminum phthalocyanine (AlPcOH(SO ) ) utilizing two custom-built red light sources. The treatment eliminates PrP signal in infectious mouse brain homogenate with efficiency that depends on light intensity, but has a low effect on the overall protein content. Importantly, singlet oxygen (O ( Δ )) is the only species significantly photogenerated by AlPcOH(SO ) , and it is responsible for the PDI of prions. More intensive light conditions show not only higher O ( Δ ) production but also decreases in AlPcOH(SO ) photostability. Our findings suggest that PDI by AlPcOH(SO ) -generated O ( Δ ) represents a promising approach for prion inactivation that may be useful in future decontamination strategies for delicate medical tools. This article is protected by copyright. All rights reserved.

DOI 10.1002/jbio.201800430
ISSN 1864-0648
Citation Kostelanska M, Freisleben J, Hanusova ZB, Mosko T, Vik R, Moravcova D, et al. Optimization of the photodynamic inactivation of prions by a phthalocyanine photosensitizer: the crucial involvement of singlet oxygen. J Biophotonics. 2019:e201800340.

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