Peroxidase Mimic Activities of Copper Selenide (CuSe) Nanoplates for Sensing H₂O₂ and L-Cysteine.

Author(s) Maojuan, B.; Chengcheng, X.; Xuanye, H.; Yanan, L.; Jun, W.
Journal J Nanosci Nanotechnol
Date Published 2020 Sep 01

Compared with natural enzymes, artificial mimic enzymes have been widely studied for their high stability and cost effectiveness. In this study, CuSe nanoplates as a simulated enzyme which does not contain precious metals, has peroxidase activity. CuSe nanoplates were prepared and characterized by powder X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and X-ray energy dispersive spectrometer (EDS). Kinetic studies show that CuSe nanoplates exhibits a higher affinity for 3,3',5,5'-teramethylbenzidine (TMB) than horseradish peroxidase (HRP). The rapid colorimetric determination of H₂O₂ and L-cysteine were developed based on the catalytic efficiency. The linear range of detection for H₂O₂ is 5.0×10~8.0×10 M, and the detection limit is 2.9×10 M, while the relative standard error is less than 5%. In addition, L-cysteine was detected with a detection limit of 0.2×10 M. The good selectivity of the determination to H₂O₂ and L-cysteine in aqueous solution was also achieved. CuSe nanoplates as a simulated enzyme for sensor applications would be used in environmental monitoring and biomedical analysis.

DOI 10.1166/jnn.2020.17856
ISSN 1533-4899
Citation J Nanosci Nanotechnol. 2020;20(9):53695375.

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