Ribonuclease H Enzyme Activity Detection Based on Hybridization Chain Reaction Amplification and Graphene Oxide Nanosheets Fluorescence Quenching.

Author(s) Liu, C.; Tang, Y.; Chen, Y.; Deng, J.; Li, W.; Tang, J.
Journal J Nanosci Nanotechnol
Date Published 2020 Mar 01

Ribonuclease H (RNase H) plays key roles in HIV virus replication process and can be used as an important target for anti-AIDS drugs screening. Therefore, we constructed a fluorescent nanosensor based on signal amplification of hybridization chain reaction (HCR) and fluorescence quenching of graphene oxide (GO) nanosheets. The nanosensor provided highly sensitive and selective platform for RNase H activity detection. This method also exhibited a good linear relationship in the range of 0.001-5 U/mL, and the detection limit was 0.0007 U/mL. Our results suggested that the developed system is a promising platform for monitoring the RNase H activity, showing great potential in the biomedical studies and drugs screening.

DOI 10.1166/jnn.2020.17351
ISSN 1533-4899
Citation J Nanosci Nanotechnol. 2020;20(3):14091416.

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